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10
- 供应商:
北京柏瑞图科技有限公司
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1Kit
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
dense products have been devised. A chromogenic substrate, 5-bromo-4-chloro-3-indolyl phosphate (X-Phos), which is very similar to X-gal, can be employed for detection and leads to production of a blue precipitate (Figure 1). In addition, the tetrazolium
Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli
as blue colonies when plating onto an agar medium containing a chromogenic substrate for PhoA. The presence of an intact PhoA signal peptide yields to a systematic secretion of the fusion proteins into the periplasm where the PhoA dimerises to its active
Dual-Color ELISPOT Assay for Analyzing Cytokine Balance
to IL-2 secreting cells (Th1) were developed with horseradish peroxidase and amino-ethyl-carbazole/H2 O2 . The light blue spots corresponding to IL-4-secreting cells (Th2) were developed with alkaline phosphatase and Vector blue (chromogenic substrate
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