- 询价
- Zymo Research
- E1010-250U
- 2026年02月19日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
250U
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文献和实验on specific amplification of cDNAs by PCR to monitor or quantify gene expression require RNA preparations devoid of any DNA contamination. For these applications digestion of contaminating DNA is recommended. This protocol makes use of Quiagen kits
DNase I Footprint Analysis of Protein‐DNA Binding
I (DNase I) protection mapping, or footprinting, is a valuable technique for locating the specific binding sites of proteins on DNA. The basis of this assay is that bound protein protects the phosphodiester backbone of DNA from DNase I?catalyzed hydrolysis
DNase I Treatment Of Total RNA
Reagents1 U/ul DNase I from Epicentre Technologies10X DNase I buffer （200 mM Tris, pH 8.4, 20 mM MgCl2, 500 mM KCl）For 1ml:200 mM Tris, pH 8.4200 ul 1M Tris, pH 8.4500 ul 1M KCl20 ul 1 M MgCl2280 ul DEPC water25 mM EDTARNeasy Kit
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