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文献和实验RUVKUN Antibody Staining Protocol revised 10/29/90
RUVKUN Antibody Staining Protocol revised 10/29/90 Fixation: 1. C. elegans N2 grown in liquid or plates. Harvest worms. Wash for 2 hours at room temp. in 1X M9 or PBS. 2. Fix in 5 ml of FRESH THAT DAY 1% paraformaldehyde:
+ Glycine (1M) + 5% normal goat serum (1hr) prior to incubating overnight (r.t) with anti-BrdU or a combination of anti-BrdU and other antibodies. Following the incubation overnight wash in 0.1M PBS (pH 7.4) with 1% TritonX100 (3x 5min) Samples
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
or possibly by immunoprecipitation and assay. In this chapter, we demonstrate the use ofthe different approaches to measure GSK3 activity in SH-SY5Y cells, describe the best antibodies currentlyavailable, and discuss the potential drawbacks of each method
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