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文献和实验Real-Time Primer Design for DNA Chips
is extensively used. The selection of the primers, which are immobilized on the DNA chip, requires a complex algorithm. Based on several parameters an optimized set of primers is automatically detected for a given gene sequence.This paper describes a parallel
Chromatin Immunoprecipitation (ChIP)
-deficient cells. Surprisingly, ~10% of the candidate sites that exhibited strong signals in the original ChIP-chip analysis, as well as in follow-up ChIP experiments performed with individual PCR primer pairs, yielded positive results of similar
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
dilution). Multiple ChIP reactions can be performed from one 3C ligation reaction. The same ligation reaction can be split and used for ChIP analyses with different antibodies. 24. Add 4-10 µg of the antibody of choice
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