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文献和实验ELISPOT (Enzyme-linked ImmunoSPOT)
PBMCs activated with PMA/Ionomycin for 24 hrs. Reference Tables Table 1: Mouse ELISPOT Ready-Set-Go! Sets & Antibody Selection Quick Guide Mouse ELISPOT Ready-Set-Go! Sets & Antibody Selection Quick Guide
The enzyme-linked immunospot (ELISPOT) assay was originally developed for the detection of individual antibody secreting B-cells. Since then, the method has been improved, and ELISPOT is used for the determination of the production of tumor
A Cell-Detachment Solution Can Reduce Background Staining in the ELISPOT Assay
-lβ, IL-2, IL-4, IL-5, and IL-8 and mouse IL-4, IL-6, and TNF-α ELISPOT assays, allowing one to remove a large number of adhered cells without hindering ELISPOT assay performance. However, Accumax was incompatible with human IFN-γ, mouse IFN-γ, canine
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