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- Protein Ark
- MD12-406-50ml
- 2026年02月19日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
50ml
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文献和实验pellet in half the required volume of freezing media. (eg if freezing 10 vials, add 5 mls freezing media) 6) Keep cell suspension on ice. 7) Slowly add dropwise with mixing an equal volume of DMSO solution. 8) Dispense 1 ml of final mixture
with mixing an equal volume of DMSO solution. 8) Dispense 1 ml of final mixture per sterile Nunc freezing vial. 9) Freeze in liquid nitrogen plug or in controlled rate Planar freezer. Freezing media 60 ml RPMI1640 or DMEM 40 ml Gammaglobulin
nitrogen container Water bath: should be maintained at 37° C 50 mL tubes 10% FBS-DMEM Petriplates Procedure Freezing Harvest MEF cells by typsinizing from petripaltes and centrifuging in 20 mL DMEM
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