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文献和实验Preparation and purification of biotin-labeled probe for ISH
; and electrophorese the sample on a mini-gel with a DNA size marker. The size of the nick translation product can be adjusted by adding a different amount of DNase I in the reaction solution. Random primer labeling also can be used for biotin-labeling
Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae
majority of cases cultured insect cells have been the host of choice. A low cost alternative to bioreactor-based protein production exists in the use of live insect larvae as “mini bioreactors.” In this chapter, we focus on Trichoplusia ni as the host
Joint Actions of Baculoviruses and Other Control Agents
pomonella ), gypsy moth (Lymantria dispar ), corn earworm (Helicoverpa zea ), tobacco budworm (Heliothis virescens ), beet armyworm (Spodoptera exigua ), and the cabbage looper (Trzchoplusia ni ) in the United States; the rhinoceros beetle (Oryctes
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