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- 库存:
10
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北京柏瑞图科技有限公司
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100ml
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文献和实验Purification of Antibodies Using Protein A-Sepharose and FPLC
1. Protein A has been expressed in recombinant form, and its crystal structure has been solved (4 ). The affinity of protein A for Fc regions is very high (K d = 10−7 ). The molecule contains four binding sites, but only two Fc domains can be bound
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G
Coupling Peptides To Resin For Affinity Purification
such peptides in 20% buffer, 80% DMSO. In an extreme case you can use 100% DMSO containing triethylamine. The amount of peptide to add is a question. We usually add 1-2 mg peptide/ml resin, hoping to make a resin that will bind 10-100 mgs/ml of specific antibody
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