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- Pluriselect
- 43-10030-40-pcs
- 2026年02月19日
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- 库存：
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- 供应商：
北京柏瑞图科技有限公司
- 规格：
pcs
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
volume of average-sized sample. Add 0.5 vol. 8 M LiCl, mix and stand at 4°C overnight. 10. Spin at 12000g, 4°C, for 15 min in a fixed angle rotor. Thoroughly resuspend pellet in 4 ml sterile gd (glass distilled) H2 O with pasteur pipette. 11. Extract twice
sec. Centrifuge the lysate at 12,000 g for 10 min at room temp. Remove the glob of bacterial debris with a sterile toothpick. Add to the supernatant 40 µl of 2.5 M NaOAc (pH 5.2) and 420 µl of isopropanol. Mix
by vortexing for 3 sec. Place the tube in a boiling water bath for exactly 40 sec. Centrifuge the lysate at 12,000 g for 10 min at room temp. Remove the glob of bacterial debris with a sterile toothpick. Add to the supernatant 40 µl of 2.5 M NaOAc (pH
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