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- 详细信息
- 文献和实验
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- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
150 ul
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文献和实验(4 ,5 ). Pinkel et al. (1988) used in situ hybridization of 120 fluorescent DNA probes (FISH) along the entire length of chromosome 4 to generate a specific painting probe for this chromosome (6 ). By the early 1990s, whole chromosome painting probes (wcp
(Vector) Biotinylated goat anti-streptavidin (Vector) Procedure Day 1: Prepare cells and DNA A. DNA: Prepare BAC DNA probe on Autogen machine (see separate protocol) Run 4-6 Autogen tubes per BAC. Hyb buffer: 50
TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs
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