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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
5 x 6 nmol
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文献和实验system. 3. SIFs and SACS Trisodium citrate was supplied by Spectrum. Silver nitrate and glucose were from Sigma-Aldrich. Microscope slides were coated by EMF Corp. (Ithaca, NY). A 52-nm thick layer of silver was deposited on the slide
absorbance at 260 nm (A260 ). Dilute the RNA solution in resuspension buffer or water, mix well. Measure the A260 of the dilution in a spectrophotometer blanked against dilution buffer (using a cuvette with a 1 cm optical path length). Calculate RNA
Chromatin immunoprecipitation on native chromatin from cells and tissues
, and place on ice for 10 minutes. Prepare two new 14mL polypropylene tubes containing each 8mL of ice-cold Buffer III. Carefully layer 2mL of each cell suspension (from step 4) on each 8mL sucrose cushion. Cover the tubes with a piece of Parafilm®
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