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- 文献和实验
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- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
500ml
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文献和实验DNA Extraction Protocols Using Silica
hood using PCR-free chemicals and supplies.Materials needed (suggested stocks are sufficient for several hundred "extractions"): Silica - ~100 g, Sigma # S-5631 GuSCN - ~500 g, Amresco (cheaper) # 0380 or Sigma # G-6639 Triton X-100
Silica Suspension: add 2 g of silica to 15 ml of H2O wash 3x by centifugation at 2000 x g for 2 min estimate vol of silica and resuspend in 2 vol H2O Silica Wash Solution: 50 mM NaCl,10 mM Tris 7.5,2.5 mM EDTA,50% Ethanol 6 M NaI note
Purification of Genomic DNA Using PureLink Silica Columns
different tubes. Before Starting Add 40 ml 96-100% ethanol to 10 ml Wash Buffer (W5) included with the kit. Store the Wash Buffer (W5) with ethanol at room temperature. Binding DNA 1. Remove a PureLink™ Spin Cartridge
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