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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
250mg
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文献和实验briefly. Then add 25 ul of 10% SDS and 5 ul of proteinase K (20 mg/ml H2O) (Sigma P-0390), vortex briefly and incubate for 1 hour at 55degC. 6. Add 120 ul phenol/chloroform/isoamyl alcohol and vortex for 30 seconds. Centrifuge the sample for 2 minutes
with about 2-nm chromium undercoat. The silver films were protected with 5 nm layer of silica. Poly-l-lysine solution was freshly prepared solution: 8 ml water 1.0 ml Na–phosphate buffer, 50 mM, pH 7.4, 1.0 ml poly-l-lysine solution (0.1%, Sigma). 实验
A Proteomics Approach to Identify Redox-Sensitive Proteins
实验试剂 1. Arabidopsis suspension culture: T87 suspension culture (RIKEN BioResource Center). 2. Liquid medium for T87 cells: 1/2 MS (Murashige and Skoog) salts, 3% sucrose, 0.29 g/L KH2 PO4 , 0.2 mg/L 2, 4-D, and B5
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