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- nanoComposix
- SIAN120-25M-25ml
- 2026年02月19日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
25ml
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文献和实验briefly. Then add 25 ul of 10% SDS and 5 ul of proteinase K (20 mg/ml H2O) (Sigma P-0390), vortex briefly and incubate for 1 hour at 55degC. 6. Add 120 ul phenol/chloroform/isoamyl alcohol and vortex for 30 seconds. Centrifuge the sample for 2 minutes
Competent cells, calcium chloride method, E. coli, description
1/100 - 1/200 to 25, 50 or 100 ml 2 X YT broth in flasks 5 - 10 X culture volume (i.e. 25 ml in 250 ml flask etc.). Grow to early log phase (OD600 = 0.2 - 0.4) (90 - 180 min depending on the strain) Collect cells by centrifugation
Preparation of Carboxypeptidase Y and Its Properties
⑸ Ampholyte (pH3～10) ; Fetal bovine serum (FBS) ⑹ 0.5mmol/L Benzyloxycarbonyl-L-phenylalanyl-L-leucine (CBZ-Phe-Leu) : Weigh 5.17mg of CBZ-Phe-Leu，dissolve it into 0.05mol/L pH6.5 phosphate buffer solution and set the volume at 25ml.
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