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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
1,000 Units at 10 U/μL
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文献和实验-stop PCR only; see Steps 1-22)SSCP loading dye (for SSCP only; see Steps 23-37)95% (v/v) formamide10 mM NaOH0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanolTaq DNA polymerase (5 U/µl)TBE Buffer (1X and 5X)Prepare a 5x stock solution in 1 liter
Obtaining Embryos: hCG Injection
for comments about fertilization rates). The hCG (Chorulon) comes as a powder of 10,000 units that we resuspend in 10 ml of sterile water (final concentration 1 unit/microliter). For the priming dose, we dilute the stock of hCG ten fold and inject 100 µl
Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
Treatment 1. Add 20 μl of 10X CIP Buffer. 2. Add diluted CIP stock to the DNA such that the final concentration is approximately 0.2 Units CIP per μg DNA in a final volume of 200 μl (see Hint #4). 3. Incubate at 37°C
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