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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
10 rxns
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文献和实验Buffer TfbI, pH5.8 500ml 1.47g KOAC 4.95g MnCl2 3.73g KCl 0.74g CaCl2
Preparing chemically competent cells
Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical flask 200μl pipetman or repeating
Transformation Ultra-Competent E. coli
Transformation "Ultra-Competent" E. coli (Inoue Method) Inoue, H., H. Nojima, and H. Okayama. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96 :23-28. Citation Abstract Procedure
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