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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
50,000 U
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文献和实验能够非常有效的抑制基因的表达。Figure 1. Silencing Gene Targets by RNase III Derived siRNA Cocktails. A 200 bp dsRNA (15 µg) for each gene of interest was digested with 2.5 U RNase III for 1 hour at 37℃. 1A. RNase III efficiently digests dsRNA . One microgram
can be used as a convenient, noncarcinogenic alternative to DEPC for treating small volumes of precious reagents and solutions such as Tris and MOPS that cannot be DEPC treated. The reagent is supplied as a 25X stock. After the RNAsecure stock is diluted
transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32P]UTP 1 µl T7 RNA
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