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- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
10 Plate
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文献和实验Protein detection onto PVDF membranes
protein microsequencing. However, the application of these techniques to proteins would not have been possible without the development of complementary detection methods. Amido Black, Coomassie Brilliant Blue R-250, colloidal gold and Ponceau S
Protein Detection Using Reversible Metal Chelate Stains
Electrophoretically separated proteins may be visualized using organic dyes, such as Ponceau red, Amido black, fast green, or most commonly Coomassie Brilliant Blue (1 ,2 ). Alternatively, sensitive detection methods have been devised using
SDS agarose gels for analysis of proteins
. The system consists of a highly resolving agarose, MetaPhor XR (FMC BioProducts, Rockland, ME, USA) dissolved in urea and TBE buffer and a stacking gel composed of a high gel-strength agarose, SeaKem Gold (FMC BioProducts). TBE containing sodium dodecyl sulfate (SDS
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