- 询价
- Immunologic
- DPVR001AP-1,0ml
- 2026年02月19日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
1,0ml
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文献和实验Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
Testing Rabbit Bleeds By Elisa
antibody diluted in Blocking Buffer for 2 hr at RT. I do tripling dilutions beginning at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP
from direct light, use within 5 min. Second stain: Double indirect immunohistochemistry (e.g rabbit anti antigen X, goat anti-rabbit AP, rabbit anti-goat AP) A second blocking step is omitted, because blocking is due to the first stain.
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