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- 2026年02月19日
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- 文献和实验
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- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
25 tests
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文献和实验microliters of 0.5M EDTA, 20 microliters 1M Tris-HCl, pH 6.5 and 2 microliters of 10mg/ml Proteinase K to the combined eluates and incubate for one hour at 45�C. 14, Recover DNA by phenol/chloroform extraction and ethanol precipitation. Addition
Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
-end ligation (1 ). The Invitrogen (Carlsbad, CA) TA Cloning� Kit combines the efficiency of cohesive-end ligation with the ease of direct cloning of PCR products. A sample may be removed from the completed PCR reaction and transferred directly
Chromatin IP (CHIP assay) This protocol has some minor modification to the protocol described in Strahl-Bolsinger S. et al. [1997, Gen & Dev 11, p83-93] and was obtained from Flick K. (The Scripps Research Institute).
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