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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
1000 mL
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文献和实验as before and then add 100 µl substrate to each well. For AP conjugates: Substrate solution is p - nitrophenyl phosphate (Sigma) at 1 mg/ml in substrate buffer. Allow the color to develop for 100 minutes and stop reaction by adding 10 µl of 1 N NaOH. Read absorbance
%) pH 7.3 AP Substrate Buffer: 0.05 M sodium carbonate-0.001 M MgCl 2 (pH 9.3) HRP Substrate: Mix equal parts TMB peroxidase substrate and TMB peroxidase soln B. Notes : 1. Sodium Azide cannot be used
with TS for 15 minutes, once with TSN for 15-20 minutes and finally once with TS for 15 minutes. 8. Develop color using the appropriate substrate: For HRP conjugates, mix 5 parts TMB peroxidase substrate, 5 parts TMB solution B, and 1 part TMB membrane
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