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- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
1 plate
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文献和实验of applications. We have developed a protocol to make ready-to-transfect DNA microarrays in a 96-well microplate for co-transfection of two plasmids into HEK293T cells. This cell-based microarray in a microplate may be used for screening hundreds of analytes
. Afterwards, the oligonucleotides are immobilized on an avidin-coated microplate, where the incorporation of [3 H]-labeled methyl groups into the DNA is stopped by addition of unlabeled AdoMet to the binding buffer. Separation of radioactively labeled DNA from unreacted AdoMet
ELISpot assays employ the sandwich enzyme-linked immunosorbent assay (ELISA) technique. Either a monoclonal or polyclonal antibody specific for the chosen analyte is pre-coated onto a PVDF (polyvinylidene difluoride) -backed microplate
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