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10
- 供应商:
北京柏瑞图科技有限公司
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文献和实验E.Z.N.A.® Yeast Plasmid Mini Spin Protocol
实验试剂 1. Absolute ethanol (96%-100%) - Do not use other alcohols 2. 2-mercaptoethanol 实验设备 1. Tabletop micro-centrifuge and nuclease-free 1.5 ml tubes 2. Water bath set to 30°C
E L I S A w i t h p l a t e l e t s
2 % for 5 min, RT wash 3 times in PBS-tween 20, dry block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC wash 3 times in PBS-tween 20, dry incubated at 37 ѓc
Wash with 500 μl Wash-Buffer Wash with 500 μl TE Add 100 μl of elution buffer. Gently pipet up and down two or three times in order to dislodge beads from the filter. Incubate 10 min in a 65 °C water bath. A water bath is used
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