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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
500 ml
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文献和实验ml bottles) for 15 min. 4. Pull off serum by aspiration (peelet is soft and will not stick to bottle). 5. Filter serum successively through 1.2 µm, .85µm and .65 µm filters using 142 mm filter holder. 6. Adjust pH to 7.0 using 1N NaOH. 7. Heat
Adult cattle serum as supplemental nutrient in lymphocyte cultures for chromosomes studies
/v) drop by drop. This medium is filtered through 0.22-micron membrane and distributed as 4 ml aliquot in 25 culture bottles, which are divided in five lots. The five lots (1-5) are supplemented with serum of five test animals (1-5) respectively
果蝇细胞的培养实验方法(Culturing Drosophila cells)
) ***[S2R+ and Kc cells will not grow in Schneider's medium from Sigma] 50 ml Fetal Bovine Serum (JRH #12103-78P) - Heat Inactivated (aliquoted in -20) 5 ml 1:100 Penicillin-Streptomycin (Gibco #15070-063) (aliquoted in -20) Sterile filter
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