- 询价
- DB Biotech
- DB Retris - 500-500 ml
- 2026年02月19日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
500 ml
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文献和实验We describe a standardized method of fixation, antigen retrieval, and image contrasting for post-embedding immunoelectron microscopy. Tissues are fixed with formaldehyde solutions containing Ca2+ and Mg2+ ions at pH 7.4 and then at pH
of the same tissue section are incubated in the enzyme solution for 10, 15, 20, 25, and 30 minutes before being immunohistochemically stained to evaluate optimum antigen retrieval time for the particular antibody being used. vi) Tap water stops the enzymatic
.3. Place sections in pronase solution at room temperature and incubate for pre-determined optimum time (approximately 10 minutes).4. Wash in TBS and proceed with stainingHeat mediated antigen retrievalHeat mediated antigen retrieval may be used
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