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- 文献和实验
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- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
1 x 500 µg
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文献和实验Immobilization of Enzymes on Magnetic Beads Through Affinity Interactions
in analytical assay because magnetic beads provide a relative large numbers of binding sites for biochemical reactions resulting in faster assay kinetics. This chapter describes immobilization procedures of tagged enzymes onto various magnetic
Efficient tagging methodologies are an integral aspect of protein complex characterization by proteomic approaches. Owing to the very high affinity of biotin for avidin and streptavidin, biotinylation tagging offers an attractive approach
(GST)或者是 GST fusion protein包含了Cdc42/Rac binding domain (CRIB) of PAK1 immobilized on glutathione–Sepharose 4B beads. The beads 用 lysis buffer清洗三次, bound GTP-Rac1 采用Rac1抗体进行免疫印渍分析 就我目前的了解来说,Rac和Cdc42都是可以采取这样的方式来检测其活性的.
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