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- 2026年02月19日
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10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
100 tests
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Interaction of p53 with Cellular Proteins
until these interacting proteins are fully characterized. This chapter outlines two methods for identifying and characterizing p53-binding proteins: (i ) glutathione-S-transferase (GST) protein—protein interaction assay; and (ii ) co-immunoprecipitation (co-IP) assay
Combine actin + GST-fusion protein and incubate for 1 hr. x 4¡C. Add 20ul 50% glutathione agarose equilibrated into binding buffer. Incubate 15 min x 4¡C with frequent mixing. Wash beads 4 times with binding buffer, spin down 4-8000 rpm
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