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文献和实验The Analysis of Mitochondria and Mitochondrial DNA in Human Embryonic Stem Cells
As human embryonic stem cells (hESCs) undergo differentiation, they express genes characteristic of the lineage for which they are destined. However, fully differentiated individual cell types can be characterized by the number of mitochondria
The Analysis of tRNA Import Into Mammalian Mitochondria
Ribonucleic acid (RNA) import into mitochondria occurs in a variety of organisms. In mammalian cells, several small RNAs are imported in a natural manner; transfer RNAs (tRNAs) can be imported in an artificial way, following expression
Measuring DNA Precursor Pools in Mitochondria
than that for the nuclear genome, and dNTP concentrations are known determinants of DNA replication fidelity. Second, several human mitochondrial diseases involve perturbations of nucleotide metabolism, and dNTP pool analysis can help us to understand the consequences
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