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文献和实验Highly parallel hybridization of nucleic acids on glass slides has successfully been applied to measure RNA and DNA abundances in Escherichia coli (1 –4 ). In this chapter, we summarize our experience in working with E. coli DNA microarrays
H-Thymidine Uptake by Cultured Cells
of labeling, synthetic rate, etc.). Thus extra control slides are always included to allow repeated sample developing until a useful number of silver grains have accumulated. Pass the slides through two changes of distilled
as described earlier (25 ). Briefly, mouse IgG was non-covalently immobilized on the sample slide, or rabbit IgG on the control slide. Slides were covered with 20 μg/ml IgG solutions in 50 mM Na-phosphate buffer (pH 7.3) and incubated at room temperature
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