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文献和实验Hematoxylin and Eosin (HE) Staining
Reagents Stains (change after every 3rd use) Harris hematoxylin: 1X stock. Anatech Lmtd. Cat #842. (616) 964-6450 Gill's Hematoxylin: See recipe. Eosin: 1x stock. Anatech Cat #837. Solutions (make fresh
Hematoxylin and Eosin Staining
Reagents Stains (change after every 3rd use) Harris hematoxylin: 1X stock. Anatech Lmtd. Cat #842. (616) 964-6450 Gill's Hematoxylin: See recipe. Eosin: 1x stock. Anatech Cat #837. Solutions
This is the most conventional stain for formalin fixed paraffin sections. The hematoxylin stains negatively charged nucleic acids (nuclei and ribosomes) blue. The eosin stains proteins pink. The hematoxylin or the eosin can also be used by themselves in more dilute form
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