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文献和实验Bromodeoxyuridine Staining of Mammary Tissue
and incubate sections overnight at 4oC with anti-BUdR-POD antibody (Boehringer Mannheim Cat.# 1 585 860) diluted 1:15 in PGB diluent. 11. Rinse sections 3 x 5 min in PBS. 12. Incubate with unactivated DAB for 5 minutes followed by activated DAB for 10 minutes
Wholemount staining of embryos
as above 15 to 30''. Secondary antibody is added in blocking solution. Concentration varies, but 1/500 works for most. For monoclonal primaries use anti-mouse coupled to AP or HRP, for polyclonals use anti-rabbit coupled AP or HRP. Incubate at 4 degC
Bromodeoxyuridine Staining of Mammary Tissue
with anti-BUdR-POD antibody (Boehringer Mannheim Cat.# 1 585 860) diluted 1:15 in PGB diluent. 11. Rinse sections 3 x 5 min in PBS. 12. Incubate with unactivated DAB for 5 minutes followed by activated DAB for 10 minutes at room temperature in the dark
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