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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
500 ml
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文献和实验probes diethyl pyrocarbonate (DEPC) and potassium permanganate can access and react with the entire sequence of the DNA, distinguishing DNA distortions “ under the foot” of a typical footprinting experiment. Combining the information obtained
1. Bring sections to water. 2. Acidified potassium permanganate 2 minutes 3. Wash in water 4. 1% Oxalic acid 1 minute 5 Wash in water 6. Wash in 70% alcohol (optional) 7. Stain in Weigerts Resorcin Fuchsin at room temperature
Detection of Mutations in DNA by Solid-Phase Chemical Cleavage Method: A Simplified Assay
in time. The method employs two commercially available chemicals, hydroxylamine (7 ) and potassium permanganate (8 –10 ) to react with unmatched cytosine and thymine, respectively. The modification of the mismatch is then followed by cleavage
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