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- ScyTek
- PBS010-10 L
- 2026年02月19日
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- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
10 L
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文献和实验Calcium Phosphate Transfection of Neurons in Primary Culture
aliquot 2X Hepes-Buffered Saline (HeBS) to second tube (150ul, in this case) add DNA/CaCl2 to 2X HeBS dropwise with pipetman, while swirling 2X HeBS let ppt form in dark, 25', room temp
Establishment of a Primary Culture
Phosphate buffered saline (PBS) Trypsin, cold sterilized in a 125 ml sterile erlenmeyer containing a magnetic stirring bar Minimum Essential Medium Fetal Calf Serum Clinical centrifuge with sterile capped
.14 Just to check enzyme and color product put 100 µl of each in a test tube and watch for a yellow color change.Figure 1. Schematic of an ELISAMATERIALSPhosphate-buffered saline (PBS) ,0.01 M Na2HPO4,0.15 M MaCl, PH 7.4Sodium phosphate dibasic (Na2HPO
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