Tyrosinase (Melanoma Marker);

利用人工组合转录因子对人类基因组扫描

) A431 cells. At 48 h after infection, these cells were stained with ten different primary antibodies (5 g/ml) specific for different cell surface markers: anti-CD15 (clone 2F3; BD, PharMingen, San Diego, CA), anti-ERBB-2 (clone SP77; ref. 5), anti-ERBB

LINEAGE ANALYSIS USING RETROVIRAL VECTORS

as one often can see macroscopic aggregates after resuspending pellets of virions. Thus, when the 2-marker approach is used to analyze clonal relationships, it is best to co-concentrate the 2 vectors together in order for the assay to be sensitive to aggregation due to this aspect

ELISPOT 发展历史

8(+) T-cell frequencies by the Elispot assay in healthy individuals and in patients with metastatic melanoma immunized with tyrosinase peptide. Int J Cancer. 87(3):391-8. 　　 Moss RB, Diveley J, Jensen FC, Gouveia E, Savary J, Carlo DJ. 2000. HIV