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10
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北京柏瑞图科技有限公司
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2 x 96 det.
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文献和实验Keywords: human embryonic stem cells; mouse neural stem cells; MTT assay; 96?well plate assays; toxicology GO TO THE FULL PROTOCOL: PDF or HTML at Wiley Online
is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk
Activation and Expansion of Human Treg Cells
of Human Treg Cells Day 0: Start with 1 x 105 Treg cells in 100 μl Culture Medium in a round bottom 96 well tissue culture plate. Add 20 μl Dynabeads Human Treg Expander. Add 500 U/ml rIL-2. Day 1: Add 100 μl Culture Medium containing 500 U/ml
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