- 询价
- Hycult Biotech
- HM2018BT-50UG-50 µg
- 2026年02月19日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
50 µg
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文献和实验T-Cell Activation Using mAb to CD3
can be performed. For costimulation studies using antibodies to other antigens, a suboptimal activation with anti-CD3 may be required. To achieve suboptimal activation via anti-CD3, a 0.5-0.1µg/ml 145-2C11 antibody solution can be used. Dispense 50µl
(Invitrogen) at a 1:3 ratio (e.g. 1µl plasmid to 3µl lipofectamine). The plasmid and lipofectamine are mixed together, vortexed, pulse spun, and then 50µl of DMEM (without Fetal bovine serum, FBS) added. The entire mixture is again vortexed followed by a pulse
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
buffer (1% BSA in PBS with 0.005% Tween 20 and 0.1% sodium azide) propidium iodide (50 µ g/ml solution with 100 U/ml DNase-free RNase) Procedure Prepare the cell type of interest as a single cell suspension and wash once with cold
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