- 询价
- Hycult Biotech
- HM2156-20UG-20 µg
- 2026年02月19日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
20 µg
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文献和实验mlAdd 40 g ammonium sulfate for every 100 ml Sup. (slowly):800gStir O/N in cold room.Pour into plastic bottles. Spin at 4 ℃, 7,500 rpm for 20 min in JA-10 rotor.Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer.Discard Sup. Resuspend
analysis of gene expression (DSAGE) measures global gene transcript levels from only 1 to 2 µg total RNA by massively parallel sequencing of cDNA tags. This unit describes the construction of 21?bp cDNA tag libraries appropriate for massively parallel
T-Cell Activation Using mAb to CD3
to each well and place in a humidified 37°C, 5% CO2 incubator. Note: For an additional stimulation control, incubate cells in 3 wells with Concanavalin A at 1-4µg/ml of culture medium. Incubate for 2-4 days. Note: Proliferation of cells between days 2 and 4 gives
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