Recombinant Human IL-17
atalog Number:
101-17
Source:
Escherichia coli.
Molecular Weight:
Approximately 31.0 kDa, a disulfide-linked homodim
er of two 132 amino acid polypeptide chains.
Quantity:
5!g/25!g/1000!g
AA Sequence:
GITIPRNPGC PNSEDKNFPR TVMVNLNIHN RNTNTNPKRS SDYYNRS
TSP WNLHRNEDPE
RYPSVIWEAK CRHLGCINAD GNVDYHMNSV PIQQEILVLR REPPHCP
NSF RLEKILVSVG
CTCVTPIVHH VA
Purity:
> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity:
Fully biologically active when compared to standar
d. The ED
50
determined by inducing IL-6
secretion of murine NIH/3T3 cells is less than 7.5
ng/ml, corresponding to a specific activity of > 1.
3
× 10
5
IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried)
powder.
Formulation:
Lyophilized from a 0.2 !m filtered concentrated so
lution in PBS, pH 7.4.
Endotoxin:
Less than 1 EU/!g of rHuIL-17 as determined by LAL
method.
Reconstitution:
We recommend that this vial be briefly centrifuged
prior to opening to bring the contents to the
bottom. Reconstitute in sterile distilled water or
aqueous buffer containing 0.1 % BSA to a
concentration of 0.1-1.0 mg/mL. Stock solutions sho
uld be apportioned into working aliquots and
stored at ≤ -20 °C. Further dilutions should be mad
e in appropriate buffered solutions.