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文献和实验RNA Isolation From Animal tissue or cell culture
empty, centrifuge the column for 2 min at full speed ($13,000 x g) to completely dry the HiBind® matrix. 11. Elution of RNA. Transfer the column to a clean 1.5 mL microfuge tube (not supplied with kit) and elute the RNA with 15-20 ul of DEPC-treated water
which provide close-ups of certain steps of the protocol. Special Feature: Complete nucleolar isolation protocol in one Quicktime movie Click here to view at high resolution (25.8 Mb) Warning
at –80°C. 3. Crush liver sample on dry ice and store in a prechilled 5 ml culture tube. 4. Add about 100 μg tissue (enough to almost cover round portion of a 5 ml culture tube) to a new chilled tube. 5. Add 1 ml PBS with 10 μl protease
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