Single-stranded DNA-binding pr

Preparation of Human Mitochondrial Single-Stranded DNA-Binding Protein

for understanding the origins of mitochondrial disease. Mitochondrial single-stranded DNA-binding protein (mtSSB) is an essential component of the mtDNA replication machinery. To facilitate ongoing biochemical studies, a recombinant source of mtSSB is needed

Expression, Purification, and In Vitro Assays of Mitochondrial Single-Stranded DNA-Binding Protein

The mitochondrial single-stranded DNA-binding protein (mtSSB) is encoded by a single-copy nuclear gene in mammals. After de novo synthesis in the cytosol, mtSSB is transported into mitochondria, where it participates in mitochondrial DNA

Family Shuffling with Single-Stranded DNA

Family shuffling, which generates chimeric progeny genes by recombining a set of naturally occurring homologous genes, is an extremely powerful approach for in vitro protein evolution. In comparison with other in vitro protein evolution methods