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文献和实验volume of 35 ml at room temperature. 2) Add the diluted buffy coat on top of 15 ml of Lymphoprep. 3) Centrifuge at 160 x g for 20 minutes at 20°C. Allow to decellerate without brakes. 4) Remove 20 ml of supernatant
from the handbook and annotated based on experience with the kit. Protocol: QIAprep Spin Miniprep Kit Using a Microcentrifuge This protocol is designed for purification of up to 20 μg of high-copy plasmid DNA from 1�5 ml overnight cultures of E. coli in LB
"Glassing Out" DNA Using the Geneclean Kit
with bound DNA by spinning in a microcentrifuge for 5 seconds at full speed (Wait for the centrifuge to come to full speed, count to five, and let it come to a halt). 9. Carefully aspirate off the supernatant and resuspend the pellet in 500 ul of NEW
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