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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
深圳子科生物科技有限公司
- 检测方法:
ELISA夹心法
- 适应物种:
小鼠
- 样本:
细胞培养上清液、血清、血浆(肝素,EDTA)和唾液
- 规格:
96 Tests
Size:96 Tests in 8 x 12 divisible strips
Assay type: Sandwich ELISA
Specificity: No detectable cross-reactivity with any other cytokine.
Storage :Store at 4°C for frequent use, at -20°C for infrequent use.
Avoid multiple freeze-thaw cycles
Shipping: Shipped on gel ice.
Expiration :Four months at 4°C and eight months at -20°C
Application: For quantitative detection of human Activin A in cell culture supernates, serum, plasma (heparin,
EDTA) and saliva.
Kit components
1. Lyophilized recombinant human Activin A standard: 10ng/tube×2.
2. 8 x 12 divisible strips precoated with anti- human Activin A antibody.
3. Sample diluent buffer: 30 ml
4. Biotinylated anti- human Activin A antibody: 130μl, dilution 1:100.
5. Antibody diluent buffer: 12ml.
6. Avidin-Biotin-Peroxidase Complex (ABC): 130μl, dilution 1:100.
7. ABC diluent buffer: 12ml.
8. TMB color developing agent: 10ml.
9. TMB stop solution: 10ml
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
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