Phospho-Met (Tyr1234/1235) Blo

Two dimensional peptide mapping

  This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over the last 20 years. Wade Gibson developed the first peptide mapping protocol

Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads

importance to employ a control to determine that the staining pattern is specific. One of the frequently used controls in IHC is a so-called absorption control: phospho-specific primary antibodies are first incubated with a phospho-peptide immunogen to block

Using Phospho‐Motif Antibodies to Determine Kinase Substrates

the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation