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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
运输温度：Shipped on Dry Ice；长期保存温度：
- 英文名：
Ras activation kit
- 库存：
50
- 供应商：
囊萤科技
- 规格：
1*30tests
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文献和实验In vitro Reconstitution of Activation of PLC by Ras and Rho GTPases
fusion protein in E. coli. Subsequently, we describe the methodology necessary to reconstitute this protein with K-Ras-4B and RhoA GTPases and measure its activation.
In situ Electroporation for the Measurement of c-Ras Activation by SVLT
between the nuclear cell cycle machinery and the membrane-signaling apparatus. Later work further revealed that Rb inactivation per se leads to Ras activation (4 ). This communication describes a novel technique to measure c-Ras activation by SVLT
, which are active in the GTP-bound form and inactive in the GDP-bound form. Given the importance of studies on the Ras protein activation state to understand the detailed mechanism of Ras-mediated signal transduction, we provide here a simple
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