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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
运输温度：Blue Ice Not Frozen；长期保存温度：+4°C
- 英文名：
IHC diluent (primary Ab)
- 库存：
50
- 供应商：
囊萤科技
- 规格：
1*250ml
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文献和实验Immunohistochemistry (IHC) of Thymus Frozen Sections
Materials Cold Acetone (-20ºC) in Coplin jars PBS, BenchKote paper, plastic lids Anti-digoxigenin-HRP diluent: 1% BSA in PBS (store 10 mL aliquots of BSA at -20ºC) Anti-rat-digoxigenin diluent: 5% Milk powder, 10% mouse serum, 10% goat
IHC frozen sections staining protocol
. If the tissue samples are fixed with an aldehyde fixative such as formalin, paraformaldehyde or glutaraldehyde and immunofluorescence (IF) is the detection method, consider including 0.3 M glycine in the blocking buffer, before applying the primary
Establishment of a Primary Culture
Establishment of a Primary Culture LEVEL III Materials Chick embryo (approximately 8 days old) 70% (v/v) ethanol for swabbing Sterile scissors, forceps and probes Sterile petri plates
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