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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
运输温度：Shipped on Blue Ice；长期保存温度：-20°C
- 英文名：
Poly-ubiquitin chains (Ub2-16) (K48-linked)
- 库存：
50
- 供应商：
囊萤科技
- 规格：
1*100µg
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文献和实验of the next one. Here we describe procedures for the synthesis of polyubiquitin chains of defined lengths that are linked through the K48 or K63 side chains. The method involves a series of enzymatic reactions in which proximally and distally blocked monoubiquitins (or chains) are conjugated
Studies of the Ubiquitin Proteasome System
( protocol 33 ), or mouse E1 ( protocol 34 ) 100 mM HEPES, pH 7.5 10× thiolester buffer (see recipe ) [32 P]ubiquitin ( protocol 26 ) or 10 µg/µl “cold
ELISPOT (Enzyme-linked ImmunoSPOT)
or controls diluted in complete RPMI-1640 medium to appropriate wells at 100µl/well. Aliquot cells at desired densities (e.g., 1x105 /ml - 2x106 /ml) at 100µl/well and incubate at 37°C, 5% CO2 humidified incubator for 24-48 hours. Note: Kinetics and cell
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