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文献和实验Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells
markers, specific for type I cells, have been described in the recent past. The first one was T1α, a 36-kDa glycoprotein found in rodent lungs. Antibodies have been developed against lung proteins that specifically label type I cells in human lung
一篇文献中的ELISA方法如下：Control peptides (14–17 amino acid sequence near the N-terminal or C-terminal of the studied protein) and the reactive rabbit anti-defensin antibodies (affinity pure) were obtained from Alpha Diagnostics .CoStar flat-bottom high
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