- 询价
- QYBIO
- 上海
- SH0213
- 2026年02月14日
企业认证
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见包装
- 库存：
不限
- 供应商：
齐一生物
- 规格：
1.0ml
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文献和实验Targeted Exon Sequencing by In‐Solution Hybrid Selection
Klenow fragment (3′→5′ exo) and Klenow buffer 10 mM dNTP mix 1 mM dATP
of oligonucleotide on a small 10% denaturing acrylamide urea gel (1 mm thick small gel ). To do this, mix equal volumes of oligonucleotide and formamide gel loading buffer (max volume for our gels is 40 microliters, but 20 microliters total is a preferred
slice solution to a Fisher screening column which has been placed in a 15 ml conical tube. Centrifuge for 1-2 min in clinical centrifuge. This step removes large pieces of acrylamide.5. Add the equivalent of 0.3 ml packed DEAE sepharose FF resin
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