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- 2026年02月14日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Protein A IgG Purification Buffer Kit
- 克隆性：
多克隆
- 保存条件：
见包装
- 规格：
3 Buffers
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
Protein A Purification of Antibody
PBS, overnight, 2-8℃.Re-equilibrate column with Binding Buffer. Store column at 2-8℃.Determine protein concentration of Ab, aliquot, and store at -20℃.3. NoteExtinction Coefficient for IgG = 1.4 and for IgM = 1.2
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