- ￥4700
- GeneTex
- 美国
- GTX77483
- 2026年02月13日
- WB, ELISA, IHC
- Goat
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Goats were immunized with purified human hemoglobin. After multiple immunizations in Freunds adjuvant the goats were bled and serum collected. The antibodies were isolated by immunoaffinity chromatography and conjugated with horseradish peroxidase
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
- 克隆性：
Polyclonal
- 标记物：
HRP
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX77483
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Goat
- 应用范围：
WB, ELISA, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Hemoglobin
- 抗体英文名：
Hemoglobin antibody (HRP)
- 抗体名：
Hemoglobin 抗体 (HRP)
- 规格：
1 mg
ELISA analysis of immunogen using GTX77483 Hemoglobin antibody (HRP).
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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Conjugation of Antibody to HRP
Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
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